on 3dfractionize

Hi, Experts,

A quick question: When I do the ROI analysis, after I decide the ROIs (either anatomical based, or based on the activation coordinates) and make the masks, should I do the 3dfractionize step before I do the ROIstats?

Is the 3dfractionize step a must?

Thanks so much for your time,

Usually you have to do 3dfractionize to match the resolution of the ROIs to your functional datasets. In my pipelines it is always a “must”. A quick look at your two datasets will tell you if they match on resolution, something like this:

3dinfo -prefix -n4 -d3 ROIs+tlrc.HEAD stats+tlrc.HEAD