FC change scores

AFNI Message Board
1

AFNI version info (afni -ver):
We created resting state functional connectivity change scores using the scripts below. We got our bilateral IFG mask from harvard oxford atlas and running appropriate scripts until 3dDeconvolve followed by the scripts below.

#    set subjectsDirectory = /Volumes/Imaging_LC/TD_change 
 #   cd ${subjectsDirectory}

    # Extract pre and post separately
   #  3dcalc \
    #  -a ${subj}_FC_newIFG_.nii.gz'[3]' -expr 'atanh(a)' -prefix ${subj}_pre_Z.nii.gz
  #  3dcalc \
 #    -a ${subj}_FC_newIFG_.nii.gz'[4]' -expr 'atanh(a)' -prefix ${subj}_post_Z.nii.gz

    # Compute change score: post - pre
#set subjectsDirectory=/Volumes/Imaging_LC/TD_change
	
 #   3dcalc -a ${subjectsDirectory}/${subj}_2_post_Z.nii.gz -b ${subjectsDirectory}/${subj}_pre_Z.nii.gz -expr 'a - b' -prefix /Volumes/Imaging_LC/${subj}_TD_change_Z.nii.gz

#end 

#set subjectsDirectory=/Volumes/Imaging_LC/ASD_change
	
 #   3dcalc -a ${subjectsDirectory}/${subj}_2_post_Z.nii.gz -b ${subjectsDirectory}/${subj}_pre_Z.nii.gz -expr 'a - b' -prefix /Volumes/Imaging_LC/${subj}_ASD_change_Z.nii.gz

#end 

#3dttest++ -AminusB -prefix /Volumes/Imaging_LC/ASDvsTD_IFG_change.nii.gz \
#-setA \
#/Volumes/Imaging_LC/ASD_change/ASD022_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD029_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD033_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD034_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD036_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD037_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD042_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD046_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD055_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD060_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD062_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD067_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD072_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD088_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD096_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD111_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD115_ASD_change_Z.nii.gz \
#/Volumes/Imaging_LC/ASD_change/ASD117_ASD_change_Z.nii.gz \
#-setB \
#/Volumes/Imaging_LC/TD_change/TD001_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD004_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD012_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD022_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD029_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD031_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD033_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD035_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD043_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD045_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD050_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD051_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD067_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD086_TD_change_Z.nii.gz \
#/Volumes/Imaging_LC/TD_change/TD088_TD_change_Z.nii.gz \

On AFNI ASDvsTD_IFG_change.nii.gz showed underconnectivity between the IFG and left postcentral gyrus.

On AFNI ASD vsTD before the intervention showed overconnectivity between the IFG and left postcentral gyrus.

On AFNI ASDvsTD after the intervention showed underconnectivity between the IFG and right calcarine gyrus.

Are we running the scripts correctly for change scores and is this typical to see- only the postcentral gyrus is appearing in the change score and not the calcarine?

Please let me know if you have any suggestions, thank you for your time!

2

Are we running the scripts correctly for change scores

From a programmatic and methodological standpoint, your scripts appear to be correctly configured to address the intended question: comparing changes in correlation between the two groups. The implementation aligns with the logic of a change score analysis.

is this typical to see- only the postcentral gyrus is appearing in the change score and not the calcarine?

Whether this observation is typical likely depends on the context of prior findings. It may be best to consult with colleagues or researchers who are more deeply familiar with the ASD literature to determine whether the involvement of the postcentral gyrus, rather than the calcarine, is consistent with established results.

Gang Chen

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