I have been trying to register some mouse brain images to the Allen Mouse Brain Common Coordinate Framework v3 brain template.
I started by converting the NRRD format to NII using the 3dSlicer software.
After that, I downsampled the Allen CCF to 75 micrometers to match our data using the 3dresample function.
Then, I tried aligning the centers of the images but the @Align_Centers function did not work.
I also tried using 3dAllineate for linear registration and auto_warp.py for non-linear registration but none of them worked.
Same steps have worked before on the template from this link:
But I didn’t have luck using the Allen mouse brain.
Would you please let me know what I need to do to make it work?"