3dfractionize with -warp option using 3dQwarp deformation

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Hello Afni Experts,
I was interested in warping an MNI ROI to subject (EPI) space. I used 3dQwarp to create a deformation field and concatenated all of the rigid/affine/non-linear files into one warp field using 3dNwarpCat. This warp file is EPI2MNI+tlrc and takes my warp into EPI resolution. This part worked out perfectly. I wanted to then pass this warp along to 3dfractionize to specify the warp from MNI to EPI subject space, this way I can also use -clip etc as some of my ROI’s otherwise leak into adjacent CSF regions when directly using 3dNwarpApply.

I tried the following:

a) 3dfractionize -template epiref.align+orig -input ROI1+tlrc -warp EPI2MNI+tlrc -vote -clip 0.2 -prefix ROI1
I keep getting an error : Warp dataset doesn’t contain a warp transformation!

b) I also tried used 3dNwarpApply to apply the EPI2MNI+tlrc warp to epiref.align+orig to create a warped version which could potentially supply the warp field.

3dfractionize -template epiref.align+orig -input ROI1+tlrc -warp epiref.align+tlrc -vote -clip 0.2 -prefix ROI1
I keep getting an error : Warp dataset doesn’t contain a warp transformation!

When using the warp field directly or a warped input it does not seem to work. Previous posts suggest using @auto_tlrc to linearly warp the ROI, however this would not really help in my case as I want to take advantage of the nonlinear warp field for more accurate ROI placement. Is there a way to circumvent this error or am I missing another step in order to take advantage of 3dfractionize’s -warp option with a nonlinear field? Or is this function limited to just affine transformation matrices?

Thanks,
Ajay

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The “-warp” option for 3dfractionize has nothing at all to do with the much-later-written nonlinear warping programs in AFNI. You’ll have to manually transform the ROI dataset back to individual subject space, and then 3dfractionize that.

I’ve almost forgotten the 3dfractionize program, which I wrote about 20 years ago. And I’d completely forgotten its “-warp” option.

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Thanks for the quick response Bob.

Best,
Ajay