3dttest++ covariates error

Hi, AFNI experts,

When I use paired 3dtest++ to analyse my group data between eyes open(EO) and eyes closed(EC) which is within subject, I want to add head motion as covariates. Therefore, I calculate averaged motion across all TRs in each subject’s condition(EC and EO). And I got 6 number for each subject’s condition,represented for 6 motion parametric(roll, pitch ,yaw,ds ,dl,dp).Then, I calculate absolute value of making EC averaged motion subtract EC averaged motion in every subject. Finally, I got a list of 6 averaged motion parametric of EC-EO for all subject. Here is the data table,which file name is ‘motion_cov_ECvEO_del03.txt’.

subj roll pitch yaw ds dl dp
subj01 0.00043366 0.0016723 0.00099257 0.0020059 0.00087426 0.00089206
subj02 0.0033435 0.0044732 0.0017124 0.0022198 0.0021446 0.003556
subj04 0.0007257 0.0014431 0.0004065 0.0064732 0.0034252 0.005052
subj05 0.00073218 0.001202 0.0013787 0.0029742 0.0015723 0.0033188
subj06 0.013967 0.016465 0.0052084 0.026858 0.01368 0.014531
subj07 0.0016505 0.0009401 0.0049 0.0096836 0.0025317 0.0043183
subj08 0.0031069 0.0039599 0.0041099 0.011722 0.0006619 0.0070911
subj09 0.0039757 0.012604 0.007849 0.036342 0.01262 0.0086569
subj10 0.0032604 0.014714 0.003251 0.0041054 0.0010881 0.0022738
subj11 0.003748 0.0030911 0.0009832 0.0077777 0.0067663 0.003505
subj12 0.0010985 0.0027555 0.0038872 0.0032114 0.0060421 0.00059851
subj13 0.0021351 0.011116 0.00054954 0.0047065 0.0021366 0.0084144
subj14 0.0045733 0.0091005 0.0035643 0.010758 0.0054094 0.0052133
subj15 8.613e-05 0.0018946 0.0014035 0.000542 0.00023811 0.001506
subj16 0.0003035 0.0091837 0.0085702 0.003258 0.0025347 0.0018505
subj17 0.0035243 0.010365 0.00025 0.0012123 0.00048712 0.0059951

And when I use this data table in 3dttest++, it occur these error messages:
++ option -setA :: processing as LONG form (label label dset label dset …)
++ option -setB :: processing as LONG form (label label dset label dset …)
++ Mixed Table: processing file /media/hjr/Elements/fMRI_berlin/result/motion_cov_ECvEO_del03.txt
** ERROR: Table: can’t open file
** ERROR: Can’t read table from -covariates file ‘/media/hjr/Elements/fMRI_berlin/result/motion_cov_ECvEO_del03.txt’
** ERROR: Try re-running this program with the extra option -DAFNI_DEBUG_TABLE=YES
** FATAL ERROR: Can’t continue after the above error!

And here is my 3dttest++ script:
3dttest++ -prefix SACC_FC_ECvEO
-paired
-setA EC subj01 ‘subj01_Z_EC_sacc+tlrc’
subj02 ‘subj02_Z_EC_sacc+tlrc’
subj04 ‘subj04_Z_EC_sacc+tlrc’
subj05 ‘subj05_Z_EC_sacc+tlrc’
subj06 ‘subj06_Z_EC_sacc+tlrc’
subj07 ‘subj07_Z_EC_sacc+tlrc’
subj08 ‘subj08_Z_EC_sacc+tlrc’
subj09 ‘subj09_Z_EC_sacc+tlrc’
subj10 ‘subj10_Z_EC_sacc+tlrc’
subj11 ‘subj11_Z_EC_sacc+tlrc’
subj12 ‘subj12_Z_EC_sacc+tlrc’
subj13 ‘subj13_Z_EC_sacc+tlrc’
subj14 ‘subj14_Z_EC_sacc+tlrc’
subj15 ‘subj15_Z_EC_sacc+tlrc’
subj16 ‘subj16_Z_EC_sacc+tlrc’
subj17 ‘subj17_Z_EC_sacc+tlrc’
-setB EO subj01 ‘subj01_Z_EO_sacc+tlrc’
subj02 ‘subj02_Z_EO_sacc+tlrc’
subj04 ‘subj04_Z_EO_sacc+tlrc’
subj05 ‘subj05_Z_EO_sacc+tlrc’
subj06 ‘subj06_Z_EO_sacc+tlrc’
subj07 ‘subj07_Z_EO_sacc+tlrc’
subj08 ‘subj08_Z_EO_sacc+tlrc’
subj09 ‘subj09_Z_EO_sacc+tlrc’
subj10 ‘subj10_Z_EO_sacc+tlrc’
subj11 ‘subj11_Z_EO_sacc+tlrc’
subj12 ‘subj12_Z_EO_sacc+tlrc’
subj13 ‘subj13_Z_EO_sacc+tlrc’
subj14 ‘subj14_Z_EO_sacc+tlrc’
subj15 ‘subj15_Z_EO_sacc+tlrc’
subj16 ‘subj16_Z_EO_sacc+tlrc’
subj17 ‘subj17_Z_EO_sacc+tlrc’
-covariates /media/hjr/Elements/fMRI_berlin/result/motion_cov_ECvEO_del03.txt
-DAFNI_DEBUG_TABLE=YES

Thanks for your help!

The first error says that it cannot open the file. Are you
sure about the path? What is the output from the commands:

ls -l /media/hjr/Elements/fMRI_berlin/result/motion_cov_ECvEO_del03.txt
file_tool -test -infile /media/hjr/Elements/fMRI_berlin/result/motion_cov_ECvEO_del03.txt

  • rick

17 subjects with 6 covariates each means you will have only 10 degrees of freedom. An effect will have to be pretty large to be seen!